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Phospho-h2ax ser139

WebProduct Specific Information. Description: The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A … WebThis Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor ® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139). Quality Evaluated by Immunocytochemistry in untreated (negative control) and Staurosporin treated HeLa cells.

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

WebFeb 2, 2024 · (D, E) IHC staining of phospho‐BRCA1 (Ser1423) and phospho‐H2AX (Ser139) on MM samples (N ≧ 7). Compared with the wild‐type, the Brca1 Mut group revealed similar fractions of EM and no malignancy, but a higher fraction of SM, as well as a lower proportion of BM (Figure 1D ), indicating the promotion of epithelial–mesenchymal ... WebAnti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated … t shirt vintage design https://energybyedison.com

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WebApr 14, 2024 · Abstract. Background: HER3, a member of the ERBB family of receptor tyrosine kinases that activates multiple oncogenic signaling pathways, is overexpressed in 50-70% of breast cancers (BC). HER3 mRNA expression is highest in luminal (ER+) breast tumors. Approximately 30% of ER+ breast tumors are de novo resistant to tamoxifen. … WebNov 15, 2024 · Cells were then incubated with phospho-histone H2A.X (Ser139) antibody (from Cell Signaling Technologies 9542, Boston, MA, USA) for 1 h, followed by DAPI … WebDescription Histone H2AX is a variant of the nucleosome core histone H2A and is phosphorylated at Ser139 in response to DNA damage. Histone H2AX phosphorylation is considered a specific reporter of double-strand DNA breaks. The protein is also referred to as γH2AX when phosphorylated at Ser139. phil steele college football preview

Alexa Fluor® 488 anti-H2A.X Phospho (Ser139) Antibody

Category:Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 - Sigma-Aldrich

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Phospho-h2ax ser139

Human Phospho-H2AX (Ser139) and Total H2AX ELISA Kit

WebAnti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p. Research Category Epigenetics & Nuclear Function Research Sub Category Histones Quality WebThis Histone H2AX phospho Ser139 antibody was raised against a peptide including phospho-serine 139 of histone H2AX. Buffer 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide. View our Guide to Histone Modifications and Biological Function. Histone H2AX phospho Ser139 antibody tested by …

Phospho-h2ax ser139

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WebNov 15, 2024 · RayBio® Phospho-H2AX (S139) and Total H2AX ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated H2AX protein in your experimental model system, you can verify pathway activation in your cell lysates. WebDescription. Histone H2AX is a variant of the nucleosome core histone H2A and is phosphorylated at Ser139 in response to DNA damage. Histone H2AX phosphorylation is considered a specific reporter of double-strand DNA breaks. The protein is also referred to as γH2AX when phosphorylated at Ser139. H2AX phosphorylation is especially strong in ...

WebAnti-phospho-Histone H2A.X (Ser139), clone 6L16 ZooMAb, is a recombinant Rabbit Monoclonal Antibody that specifically targets phospho Histone H2AX (ser139) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. Web公司专业供应的培养基,dragon牙膏稀释缓冲液使用说明书现货直销,质量有保障,款到当天可发货,免费快递送货上门,欢迎 ...

WebIntracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry. 1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C. 2. Prepare cells of interest. 3. Wash 1X with PBS, centrifuge at 350g for 5 min. 4. WebPhosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting, immunofluorescence and flow cytometry. Product Details Technical data sheet Product Details Verified Reactivity Human, Mouse Antibody …

WebMar 31, 2016 · Get better batch-to-batch reproducibility with a recombinant antibody. Anti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] (ab81299) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production.

WebFeb 11, 2024 · body mass index modifies the relationship between gamma-h2ax, a dna damage biomarker, and pathological complete response in triple-negative breast can.pdf 2024-02-11 上传 body mass index modifies the relationship between gamma-h2ax, a dna damage biomarker, and pathological complete response in triple-negative breast can philsteele.com storeWebThe AlphaLISA SureFire Ultra Phospho-Histone H2A.X (Ser139) assay kit is an immunoassay for quantitative detection of phosphorylated Histone H2A.X in cellular lysates. We are now two standalone organizations Life Sciences & Diagnostics and Analytical & Enterprise Solutions phil steele football previewWebJan 13, 2011 · Herein, we report that EGF strongly induces histone H2AX phosphorylation at Ser139 and at a newly identified phosphorylation site, Ser16, in vitro and ex vivo, and both phosphorylations are mediated by RSK2.Phosphorylation of histone H2AX by RSK2 prevented histone H2AX degradation, and histone H2AX could block cell transformation … t-shirt vintage televisionWebBecause phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA … phil steele college football preview 2018WebPhospho-Histone H2A.X (Ser139/Tyr142) Antibody recognizes endogenous levels of histone H2A.X protein only when phosphorylated at Ser139 or Tyr142, or dually phosphorylated on both residues. This antibody does not cross-react with other histone proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification t shirt vintage usaWebBethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry … t shirt vinyl cutting machineWebImmunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H2A.X (Ser139) in HeLa cells +/- Staurosporin treatment. Target description 17 kDa observed Physical form Protein G Purified Purified mouse monoclonal IgG1 in buffer containing PBS with 15 mg/mL BSA and 0.1% azide Storage and Stability phil steele georgia football